Assessment of assay performance through oligonucleotide reconstitution studies. Panels A and B: Known input percentages are indicated (grey, total 100%), measured percentages are indicated in black. Clonotype number is indicated on x-axis, arranged by descending measured percentage. All measured percentages are shown to the level of the lowest input percentage. Panel C: Summary plot of the 26 reconstitution mixes representing all 108 clonotypes. Dotted line shows correlation between input and measured percentages (R = 0.90, p < 0.0001, log10M = 100.68 *log10I + 0.28). A 45° reference line (solid) is shown.