Figure 1

Effect of total number of tags sequenced on number of distinct tag sequences identified. LongSAGE (A) and MPSS (B) libraries produced from an activated CD4⁺ T-cell clone were sampled at various sizes to examine the effect of library size on the number of distinct tag sequences identified. If the library is large enough to sample all available tags, then increasing the library size will not increase the number of sequences detected. Closed diamonds represent all tags in the library. Open circles represent only those tags that exactly match either the genome or the transcriptome (i.e. excluding possible sequencing errors but also polymorphisms and some tags crossing splice junctions).