Figure 4

Impact of 5-prime limited ESTs on the categorization of AS events. In this representative example, the alignment of EST1 to the genome sequence identifies an intron in the cognate gene. EST2 reads into this intron and thus provides evidence of the alternative splicing of this gene. However, as EST2 does not extend through the entire intron, the sequence data supports either retention of the entire intron (B₁), or that there was an alternative donor site (B₂).