Figure 4

Splice site specific SILV RT-PCR in differentially pigmented bovine skin. A. Detection of bovine SILV splice variant Δ105–362 in differentially pigmented skin. RT-PCR products generated by splice site specific RT-PCR with primers SPex23_F1 – E5_R1. M: marker; C: negative control, 1: non-pigmented skin from a heterozygous dilute (Dc^D/Dc^d), eumelaneic (E^D/E^D) spotted individual; 2: pigmented skin from a heterozygous dilute (Dc^D/Dc^d), eumelaneic (E^D/E^D) spotted individual; 3: crème white skin from a homozygous dilute (Dc^D/Dc^D), eumelaneic (E^D/E^D) non-spotted individual ; 4: non-pigmented skin from a heterozygous dilute (Dc^D/Dc^d), phaeomelaneic (E^e/E^e) spotted individual; 5: pigmented skin from a heterozygous dilute (Dc^D/Dc^d), phaeomelaneic (E^e/E^e) spotted individual; 6: non-pigmented skin from a homozygous non-dilute (Dc^d/Dc^d), eumelaneic (E^D/E^e) spotted individual; 7: pigmented skin from a homozygous non-dilute (Dc^d/Dc^d), eumelaneic (E^D/E^e) spotted individual. B. RT-PCR products generated with GAPDH primers.