Multiple splice variants within the bovine silver homologue (SILV) gene affecting coat color in cattle indicate a function additional to fibril formation in melanophores

Table 3 Splice site specific RT-PCR primer combinations applied for the detection of alternative bovine SILV transcripts

Alternative splice site	Upstream primer	Downstream primer	Expected alternative transcript [bp]
Δ105–362	SPex23_F1 TGTAGGGACCACAGAAGGGAG	E5_R1 TAATGGTGAAGGCTGAACTGG	311
Δ498–1340	SPex56_F1 TGTCTGGAAGACCTGGGGCT	E9_R2 TGACACCCTGGCGATGAGATG	264
Δ660–1340	E5_F2 GGGCACATATAACATGGAAGTG	SPex6_R1 GCAGGGGACTCAGGGAGCCAG	113
Δ816–1340	SPex6A_F1 CCTACACCTGGGACTTTGGCT	E9_R2 TGACACCCTGGCGATGAGATG	265
Δ1185–1340	SPex6B_F1 CAACTGCAAAAGCTACAGGCT	E9_R2 TGACACCCTGGCGATGAGATG	265
Δ1655–1748	E7_F2 TGTGTTCTGTATCGCTATGGCTC	SPex9_R1 CTGAGGCCTGCTTCTTGCCCTG	265

ISSN: 1471-2164