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Table 8 Properties of PCR amplicons used for microarray resequencing of chimpanzee and gorilla: size, required mass and observed concentration, and volume added to pool

From: Interspecies hybridization on DNA resequencing microarrays: efficiency of sequence recovery and accuracy of SNP detection in human, ape, and codfish mitochondrial DNA genomes sequenced on a human-specific MitoChip

Chimpanzee PCR Amplicons Size (bp) Mass (ng) Conc. (ng/mL) Volume (uL)
h01F, h10R 6736 244.5 19.8 12.35
h09F, h13R 3582 130.0 11.1 11.71
h14F, h17R 2885 104.7 5 20.95
h18F, h18R 866 31.4 9.4 3.34
h19F, h19R 976 35.4 6.9 5.13
h20F, h22R 2679 97.2 12.5 7.78
h23F, h23R 807 29.3 11.1 2.64
Gorilla PCR Amplicons Size (bp) Mass (ng) Conc. (ng/mL) Volume (uL)
h01F, h06R 4082 148.2 19.4 7.64
h06F, h09R 2888 104.8 15.1 6.94
h10F, h10R 886 32.2 14.1 2.28
Gg11F, Gg12R 1420 51.5 14.7 3.51
h13F, h13R 815 29.6 20.6 1.44
h14F, h17R 2885 104.7 22.8 4.59
Gg18F, Gg18R 590 21.4 25.4 0.84