Table 8 Properties of PCR amplicons used for microarray resequencing of chimpanzee and gorilla: size, required mass and observed concentration, and volume added to pool
Chimpanzee PCR Amplicons | Size (bp) | Mass (ng) | Conc. (ng/mL) | Volume (uL) |
|---|---|---|---|---|
h01F, h10R | 6736 | 244.5 | 19.8 | 12.35 |
h09F, h13R | 3582 | 130.0 | 11.1 | 11.71 |
h14F, h17R | 2885 | 104.7 | 5 | 20.95 |
h18F, h18R | 866 | 31.4 | 9.4 | 3.34 |
h19F, h19R | 976 | 35.4 | 6.9 | 5.13 |
h20F, h22R | 2679 | 97.2 | 12.5 | 7.78 |
h23F, h23R | 807 | 29.3 | 11.1 | 2.64 |
Gorilla PCR Amplicons | Size (bp) | Mass (ng) | Conc. (ng/mL) | Volume (uL) |
h01F, h06R | 4082 | 148.2 | 19.4 | 7.64 |
h06F, h09R | 2888 | 104.8 | 15.1 | 6.94 |
h10F, h10R | 886 | 32.2 | 14.1 | 2.28 |
Gg11F, Gg12R | 1420 | 51.5 | 14.7 | 3.51 |
h13F, h13R | 815 | 29.6 | 20.6 | 1.44 |
h14F, h17R | 2885 | 104.7 | 22.8 | 4.59 |
Gg18F, Gg18R | 590 | 21.4 | 25.4 | 0.84 |