Table 1 Novel AZFc amplicon-specific sequence family variants (SFVs)
From: Characterizing partial AZFc deletions of the Y chromosome with amplicon-specific sequence markers
SFV | GenBank accession number | AZFc reference assembly BAC mappinga, b | SFV position (bp in each BAC) | Restriction Enzyme | Restriction Fragments (bp) | Allelic variantsc |
|---|---|---|---|---|---|---|
b2_AZFc-SFV | BV686548 | AC008175 (b2) | 62.881–63.530 | Mnl I | A- 653 | +3ntd (b1, b3, b4) |
AC016752 (b3) | 80.268–80.920 | B- 452+197 | no insertion (b2) | |||
AC007965 (b4) | 99.077–99.729 | |||||
b3_AZFc-SFV | BV686549 | Same as above | 52.143–52.652 | Bln I | A- 510 | T (b1, b2, b4) |
91.161–91.670 | B- 261+249 | C (b3) | ||||
88.327–88.836 | ||||||
b4_AZFc-SFV | BV686550 | Same as above | 43.853–44.482 | Hph I | A- 630 | G (b1, b2, b3) |
99.325–99.954 | B- 347+282 | A (b4) | ||||
80.041–80.670 | ||||||
g1_AZFc-SFV | BV686551 | AC006366 (g1) | 59.421–59.920 | Bsm AI | A- 500 | C (g2, g3) |
AC010153 (g2) | 54.938–55.437 | B- 272+224 | G (g1) | |||
AC016728 (g3) | 50.071–50.570 | |||||
g2_AZFc-SFV | BV686552 | Same as above | 61.496–61.987 | Tsp 45I | A- 274+170+38 | G (g2) |
52.871–53.362 | B- 274+137+38+28 | C (g1, g3) | ||||
52.146–52.637 | ||||||
g3_AZFc-SFV | BV686553 | Same as above | 62.357–62.774 | Bsm AI | A- 400 | no insertion (g3) |
52.084–52.501 | ||||||
53.007–53.406 | B- 251+163 | +18nte (g1, g2) | ||||
Gr_AZFc-SFV | BV686554 | AC006983 (Gr1) | 158.663–159.102 | Sna BI | A- 440 | A (Gr1) |
AC024067 (Gr2) | 13.026–13.465 | B- 311+129 | G (Gr2) |