RBM6-RBM5 chimeric transcript expression. Chimeric expression was examined using nested RT-PCR on tissue or cell line mRNA. A. Expression in purchased human tissue samples: lung – large cell carcinoma; ovary – cystadenoma; skeletal muscle – malignant fibrous histiocytoma, poorly differentiated; skin – malignant melanoma; spleen – malignant lymphoma; uterus – adenocarcinoma, well differentiated. B. Expression in purchased human tissue samples: bone – osteoblastic osteosarcoma; brain – astrocytoma, moderately differentiated; lymph node – non-Hodgkin's lymphoma of tonsil; pancreas – insulinoma; prostate – adenocarcinoma, moderately differentiated; and testis – seminoma. C. Expression in three tumour samples from the Ontario Cancer Research Network, and an unmatched purchased non-tumour sample. D. Expression in human cancer cell lines: GLC20 – small cell lung carcinoma; MDA-MB-231 – pleural effusion of a breast adenocarcinoma; JKM1 – Jurkat T lymphoblastic leukemia subclone; MCF-7 – pleural effusion of a breast adenocarcinoma; TF-1 – erythroblastic leukemia; A431 – epidermoid carcinoma; HeLa – cervical carcinoma; BT-474 – invasive ductal breast carcinoma; K-562 – chronic myelogenous leukemia; Raji – Burkitt's lymphoma. NT: non-tumour tissue, T: tumour tissue.