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Table 2 PCR and sequencing confirmation of CDSs identified as absent/divergent by CGH analysis

From: Comparative genomic analysis of Tropheryma whipplei strains reveals that diversity among clinical isolates is mainly related to the WiSP proteins

     PCR and sequencing
Gene ID Gene name Gene product Intensity of Hybridization lower than with Twist Genes deleted Genes with nt variation(s) Genes conserved
TWT018 deoD purine nucleoside phosphorylase 1   1  
TWT041 - hypothetical protein 7 7   
TWT099 - hypothetical protein 3   3  
TWT101 - hypothetical protein 1 1   
TWT151 - hypothetical protein 1    1
TWT158 - hypothetical protein 3 3   
TWT171 - hypothetical protein 2   2  
TWT176 - hypothetical protein 1   1  
TWT199 ftsE cell division ATP-binding protein 5   5  
TWT203 ksgA dimethyladenosine transferase 1   1  
TWT232 - WiSP family protein 3   3  
TWT277 - hypothetical protein 1   1  
TWT311 - hypothetical protein 1   1  
TWT386 trpE anthranilate synthase component I 1   1  
TWT388 - hypothetical protein 2   2  
TWT594 - WiSP family protein 9   9  
TWT596 - WiSP family protein 7 6 1  
TWT604 - hypothetical protein 2 1 1  
TWT613 - hypothetical protein 1 1   
TWT614 ftsK cell division protein FtsK 1 1   
TWT615 - hypothetical protein 1 1   
TWT617 - hypothetical protein 1 1   
TWT618 wblE Putative transcription reg. protein 1 1   
TWT619 - hypothetical protein 1 1   
TWT621 - hypothetical protein 1 1   
TWT624 - WND-containing WiSP family protein 1 1   
TWT653 - membrane protein 2 1 1  
TWT673 - hypothetical protein 1   1  
TWT679 - hypothetical protein 3   3  
TWT704 - hypothetical protein 2   2  
TWT722 - hypothetical protein 1    1
TWT751 - hypothetical protein 1   1  
TWT762 - hypothetical protein 2   2  
TWT773 - hypothetical protein 1   1  
   TOTAL 72 (100%) 27 (37.50%) 43 (59.70%) 2 (2.80%)
  1. The values correspond to the number of strains for which mentioned parameters were found different to that observed in T. whipplei Twist. (nt): nucleotide.