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Table 2 PCR and sequencing confirmation of CDSs identified as absent/divergent by CGH analysis

From: Comparative genomic analysis of Tropheryma whipplei strains reveals that diversity among clinical isolates is mainly related to the WiSP proteins

    

PCR and sequencing

Gene ID

Gene name

Gene product

Intensity of Hybridization lower than with Twist

Genes deleted

Genes with nt variation(s)

Genes conserved

TWT018

deoD

purine nucleoside phosphorylase

1

 

1

 

TWT041

-

hypothetical protein

7

7

  

TWT099

-

hypothetical protein

3

 

3

 

TWT101

-

hypothetical protein

1

1

  

TWT151

-

hypothetical protein

1

  

1

TWT158

-

hypothetical protein

3

3

  

TWT171

-

hypothetical protein

2

 

2

 

TWT176

-

hypothetical protein

1

 

1

 

TWT199

ftsE

cell division ATP-binding protein

5

 

5

 

TWT203

ksgA

dimethyladenosine transferase

1

 

1

 

TWT232

-

WiSP family protein

3

 

3

 

TWT277

-

hypothetical protein

1

 

1

 

TWT311

-

hypothetical protein

1

 

1

 

TWT386

trpE

anthranilate synthase component I

1

 

1

 

TWT388

-

hypothetical protein

2

 

2

 

TWT594

-

WiSP family protein

9

 

9

 

TWT596

-

WiSP family protein

7

6

1

 

TWT604

-

hypothetical protein

2

1

1

 

TWT613

-

hypothetical protein

1

1

  

TWT614

ftsK

cell division protein FtsK

1

1

  

TWT615

-

hypothetical protein

1

1

  

TWT617

-

hypothetical protein

1

1

  

TWT618

wblE

Putative transcription reg. protein

1

1

  

TWT619

-

hypothetical protein

1

1

  

TWT621

-

hypothetical protein

1

1

  

TWT624

-

WND-containing WiSP family protein

1

1

  

TWT653

-

membrane protein

2

1

1

 

TWT673

-

hypothetical protein

1

 

1

 

TWT679

-

hypothetical protein

3

 

3

 

TWT704

-

hypothetical protein

2

 

2

 

TWT722

-

hypothetical protein

1

  

1

TWT751

-

hypothetical protein

1

 

1

 

TWT762

-

hypothetical protein

2

 

2

 

TWT773

-

hypothetical protein

1

 

1

 
  

TOTAL

72 (100%)

27 (37.50%)

43 (59.70%)

2 (2.80%)

  1. The values correspond to the number of strains for which mentioned parameters were found different to that observed in T. whipplei Twist. (nt): nucleotide.
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BMC Genomics

ISSN: 1471-2164

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