The small FNR regulon of Neisseria gonorrhoeae: comparison with the larger Escherichia coli FNR regulon and interaction with the NarQ-NarP regulon

Table 2 Complementation of E. coli narXLQP mutations by gonococcal NarQ and NarQP.

Host strain	Host genotype	Plasmid	Reporter	β-galactosidase activity
				Ø₂	+ NO₂ ^-	+ NO₃ ^-
A. Complementation of a narXLQP mutation
JCB386		none	nirB::lacZ	120	650	1200
JCB3861	narXL	none	nirB::lacZ	80	130	570
JCB3863	narXLQP	none	nirB::lacZ	49	39	61
JCB3863	narXLQP	pGCNarQP	nirB::lacZ	64	67	81
B. Phosphorylation of E. coli NarP by gonococcal NarQ
JCB391	narXLQ	none	napF::lacZ	130	110	120
JCB391	narXLQ	pBADgcQ	napF::lacZ	1630	1390	1350
C. Phosphorylation of E. coli NarL by gonococcal NarQ
JCB12	narXQ	none	frdA::lacZ	2830	2070	2570
JCB12	narXQ	pBADgcQ	frdA::lacZ	780	650	820

E. coli transformed with the stated plasmids was grown anaerobically in LB in A. and B. and in minimal medium in C. in the absence or presence of 2.5 mM NaNO₂ or 20 mM NaNO₃ and the β-galactosidase activities determined.

ISSN: 1471-2164