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Table 2 The top 5 most significant Gene Ontology (GO) terms significantly enriched in K-means clusters 6 to 13

From: Androgen-induced masculinization in rainbow trout results in a marked dysregulation of early gonadal gene expression profiles

KM Term (GO ID) P-Value RE (n/N)
6 cell surface (9986) 0.0000 12.30 (5/11)
  T cell activation (42110) 0.0005 16.20 (3/5)
  immune response (6955) 0.0015 4.50 (6/36)
  lipid binding (8289) 0.0020 5.20 (5/26)
  neuron development (48666) 0.0022 6.70 (4/16)
7 extracellular matrix (sensu Metazoa) (5578) 0.0000 4.60 (10/32)
  actin binding (3779) 0.0002 4.50 (8/26)
  muscle contraction (6936) 0.0004 6.70 (5/11)
  phosphate transport (6817) 0.0006 8.40 (4/7)
  structural molecule activity (5198) 0.0012 2.30 (15/96)
8 condensed chromosome (793) 0.0005 15.50 (3/5)
  response to endogenous stimulus (9719) 0.0025 5.00 (5/26)
  DNA repair (6281) 0.0061 5.20 (4/20)
  magnesium ion binding (287) 0.0087 4.70 (4/22)
  chromosome (5694) 0.0224 3.00 (5/43)
9 calcium ion binding (5509) 0.0004 2.50 (15/63)
  organ morphogenesis (9887) 0.0006 2.60 (13/52)
  plasma membrane (5886) 0.0011 2.10 (18/90)
  organ development (48513) 0.0018 2.10 (16/79)
  structural constituent of cytoskeleton (5200) 0.0020 4.80 (5/11)
10 urogenital system development (1655) 0.0060 7.00 (3/6)
  lipid binding (8289) 0.0080 3.20 (6/26)
  response to temperature stimulus (9266) 0.0100 6.00 (3/7)
  protein kinase binding (19901) 0.0100 6.00 (3/7)
  circulation (8015) 0.0152 5.20 (3/8)
11 metallopeptidase activity (8237) 0.0013 5.40 (5/12)
  proteolysis (6508) 0.0016 2.40 (13/69)
  peptidase activity (8233) 0.0022 2.50 (12/63)
  endopeptidase activity (4175) 0.0079 2.50 (9/47)
  transcription from RNA polymerase II promoter (6366) 0.0381 2.00 (8/51)
12 ligase activity, forming carbon-nitrogen bonds (16879) 0.0050 3.00 (7/21)
  metallopeptidase activity (8237) 0.0061 3.80 (5/12)
  mRNA metabolism (16071) 0.0165 2.10 (10/44)
  glutamine family amino acid biosynthesis (9084) 0.0200 4.60 (3/6)
  regulation of RNA metabolism (51252) 0.0322 3.90 (3/7)
13 steroid biosynthesis (6694) 0.0020 10.80 (3/9)
  sex differentiation (7548) 0.0028 9.70 (3/10)
  transcription, DNA-dependent (6351) 0.0050 2.30 (11/156)
  hormone metabolism (42445) 0.0063 7.50 (3/13)
  DNA binding (3677) 0.0071 2.20 (11/163)
  1. These GO terms were selected as the 5 most significant GO terms enriched in each K-means cluster (KM) 6 to 13 with a P-value < 0.05 and a relative enrichment RE > 2. n/N: number of genes assigned with a specific GO term in the cluster (n) with regards to the number of all genes assigned with the same GO term in the 2,474 analyzed genes (N). Interesting GO terms in regard to gonad differentiation and development are underlined. Genes with GO terms in bold type are detailed in figure 6. GO categories containing fewer than 5 genes were excluded.