Figure 3

Semi-quantitative RT-PCR validation of selected microarray data. Gene expression levels of CYP307A1 and CYP6M2 between females and males of the susceptible Kisumu strain were compared. (A) Peak intensities of amplified cDNA products of the two target mRNAs were measured and normalised by the internal control, the ribosomal S7 product. Primers used to amplify cDNA are shown in Table 1. (B) Measurements were taken from four biological replicates for each sex (15 one-day adult mosquitoes per replicates). Dots represent normalised peak intensities for each replicate. Horizontal bars show mean values. CYP307A1 shows a 3.4-fold over-expression in males (one-sided t-test, p < 0.001) whereas CYP6M2 was 1.1-fold over-expressed in males but statistically not significant (one-sided t-test, p = 0.3).