Transcription profiling of a recently colonised pyrethroid resistant Anopheles gambiae strain from Ghana

BMC Genomics

Table 3 Sequences of oligonucleotide primers used in semi-quantitative RT-PCR validation experiments

Gene	Accession number	Primer	Sequence (5' to 3')	Transcript length
CYP6M2	[GenBank: AY193729]	CYP6M2_F CYP6M2_R	GTATGATGCAGGCCCGTATAG GCCATAATGAAACTCTCCTTCG	112 bp
CYP307A1	[GenBank: AY745209]	CYP307A1_F CYP307A1_R	ACTACGAGCTGAACACGAGTGA ATGCAGGTCCGTTTACCAATAC	253 bp
S7 ribosomal	[GenBank: AY380336]	SPC SPD	GTGCCGGTGCCGAAACAGAA AGCACAAACACTCCAATAATCAAG	472 bp

Only genes are listed which showed at least a two-fold difference in one or both strains. p-values were corrected for multiple testing as described in the materials and methods section. n.s., p = 0.001. *CYP6Z1 was spotted on the detox chip as both cDNA and 70-mer oligonucleotide probe [16].

ISSN: 1471-2164