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Figure 2 | BMC Genomics

Figure 2

From: A method for the construction of equalized directional cDNA libraries from hydrolyzed total RNA

Figure 2

Characterization of equalized cDNA libraries. 257 cDNA sequences from the Danio rerio and Drosophila melanogaster self-subtracted cDNA libraries were used as GenBank and Ensembl database query sequences. Diagnostic properties of near identity (>97%) matches were collected and scored. The labeled columns represent the following library characteristics: mRNA – the fraction of clones matching known or hypothetical mRNA sequences. mRNA (novel) – the fraction of clones representing the first reported matches to hypothetical mRNA sequence or previously unknown splice variants of known mRNA transcription units. hnRNA – the fraction of clones matching sequence that is likely removed during mRNA processing, eg. intron. ncRNA – the fraction of clones matching known or hypothetical non-coding RNA transcription units. interspersed repetitive – the fraction of clones matching more than 50 dispersed loci in the genome with probabilities of e < 10-9. gDNA – the fraction of clones matching genomic DNA outside of any known or predicted transcription unit. no match – the fraction of clones that did not match any known cDNA and could not be assigned an origin in the genome. sense – the fraction of matches in the sense orientation of known or hypothetical transcription units. antisense – the fraction of matches in the antisense orientation of known or hypothetical transcription units.

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