Intracellular accumulation of TG in response to various FFAs. Confluent HepG2 cells were exposed to various FFA for 24 h and the intracellular TG levels were measured enzymatically after cell lysis and normalized to the protein levels. Results presented as mean +/- s.d. of 3 independent experiments. , significantly different than control (p < 0.01), # significantly different from palmitate (p < 0.05).