Effects of various FFAs on the expression of stearoyl-CoA desaturase (SCD). Cells were cultured in the presence of 0.7 mM palmitate or oleate for 24 h. (a) Effects on SCD transcript (mRNA) levels. RNA was extracted and the RT-PCR performed as explained in the materials and methods section. Results presented as transcript levels for the different conditions relative to control. Data shown as mean +/- s.d. of 3 independent experiments. , significantly different than control (p < 0.01). (b) SCD protein levels. After treating cells for 24 h with the FFAs, cells were lyzed and the protein levels of SCD were estimated using Western Blot. B = BSA medium, P = 0.7 mM, O = 0.7 mM oleate.