Effects of various FFAs on the expression of mitogen activated protein kinase kinase kinase kinase 2 (MAP4K2). Cells were cultured in the presence of 0.7 mM palmitate or oleate and 100 ng/ml TNF-α for 24 h. RNA was extracted and RT-PCR performed as explained in the materials and methods section. Results are presented as ratio of transcript levels with respect to control. Data presented as mean +/- s.d. of 3 independent experiments. , significantly different than control (p < 0.05). For the x-axis legends, the postscript '100' denotes the TNF-α concentration in ng/ml.