A. Compartmentalization of the A. gambiae midgut in 4 parts: cardia, anterior, anterior-posterior and posterior-posterior. B: Validation of microarray-assayed gene expression with real-time quantitative RT-PCR. Expression data (log2 ratio) of 11 genes from four midgut assays (male versus female; anterior midgut versus whole female midgut, anterior part of posterior midgut versus whole female midgut; cardia versus posterior midgut) obtained from microarray analysis were plotted against the corresponding expression values obtained with real time RT-PCR from the mean of the three replicates of each experiment. The best-fit linear-regression analysis (R2 = 0.7483) and the slope of the regression line (m = 1.5263) demonstrate a high degree of correlation of the magnitude of regulation between the two assays. C: Functional gene class distribution of the putative total midgut transcriptome as predicted by the pooled transcriptomes of female and male midguts, and all individual midgut compartments that were assayed in this study. D: Comparison of the functional class distribution of transcripts in the midguts of female and male mosquitoes. Transcripts enriched by greater than a 2-fold in the female and male midguts are represented by the green and red sections, respectively. Transcripts with similar abundance in midguts of both sexes are represented by the black section. The functional gene class distribution within each group is indicated by the pie charts.