Frame disruptions in human mRNA transcripts, and their relationship with splicing and protein structures

Table 5 Frame disruption placement and nonsense-mediated decay

Type of initial frame disruption	Alignment verification	NMD or non-NMD region *
		Observed [NMD : non-NMD]	Expected [NMD : non-NMD]	Significance
Frameshift	All cases	159 : 188	294.9 : 52.1	†††
	Cases with verifying alignments	122 : 159	206.6 : 74.4	†††
Stop codon	All cases	43 : 30	42.9 : 30.1	N.S.
	Cases with verifying alignments	22 : 17	17.5 : 21.5	N.S.
Frameshift or stop codon	All cases	201 : 218	344.5 : 74.5	†††
	Cases with verifying alignments	141 : 184	232.0 : 93.0	†††
	Cases with verifying alignments (without 'probable UTR features')	87 : 122	111.5 : 97.5	†††

* Ratios are for numbers expected or observed in NMD regions versus non-NMD ones. Expectations and tests are performed as for Table 1.