Confirmation of microarray results for skeletal muscle by Q-RT-PCR. The experiments were performed on samples derived from C57BL/6 male mice. The result values are expressed as mean of triplicate runs +/- standard deviation. Statistical significant differences relative to control diet fed mice were determined. *p < 0,05; **p < 0,01; ***p < 0,001. A, Q-RT-PCR analysis of 6 genes with up-regulated expression after iron overload. B, Q-RT-PCR evaluation of 5 genes with iron-induced down-regulation of expression by microarray analysis.