Verification of data obtained for heart samples by microarray analysis using Q-RT-PCR. Samples from C57BL/6 male mice were used in these experiments. The result values are expressed as mean of triplicate runs +/- standard deviation. Statistical significant differences relative to control diet fed mice were determined. *p < 0,05; **p < 0,01; ***p < 0,001. A, Q-RT-PCR evaluation of seven genes with up-regulated expression after iron overload. B, Q-RT-PCR analysis of seven genes with iron-induced down-regulation of expression by microarray.