Figure 4
From: A distinct epigenetic signature at targets of a leukemia protein
Repressive epigenetic changes at the AML1-targets. A. ChIP with antibodies against either acetylated histone H4 or tri-methylated histone H3 Lysine 9 (tri-Met-H3-K9) followed by quantitative PCR with primers amplifying a region encompassing either the transcription start site (+1) or the AML1-consensus detected a different level of repressive histone changes in all five genes in A16 cells. B. In silico analysis identified a CpG island only in the Mgmt promoter. This CpG island is hypermethylated in A16 cells (bottom, right).