Figure 2

Genomic Organization of the Human and Mouse NEAT1 and NEAT2 Loci. (A, B) Graphical representation of locations and lengths of transcripts and probes mapping to the genomic region of (A) human NEAT1/NEAT2 on 11q13.1 and (B) mouse Neat1/Neat2 on 19qA. All transcripts run 5' to 3' from left to right. Transcripts represented in green (NEAT1, NEAT2, Neat1 and Neat2) were characterized by Northern analysis, 5'RLM-RACE and 3' RACE. Genbank accession number for these transcripts are: NEAT1 – EF177379, NEAT2 – EF177381, Neat1 – EF177378 and Neat2 – EF177380. For these transcripts, all 3' poly(A) ends had a consensus AAUAAA 10–30 nucleotides 5' of the poly(A) site and a GU rich and/or U rich element 3' of the site. Human NEAT1 is observed to extend past its primary poly(A) site, resulting in a larger, >17 kb transcript. The 3' end of this larger transcript is drawn fading to represent that while its location is suggested by AF001893 (Guru et al., 1997) and our own Northern analyses (data not shown), we were unable to fully map its 3' end by RACE, most likely due to a series of genomic poly(A) repeats throughout the transcript. Transcripts in black were previously described to various levels by EST walking, Northern and RACE analysis. Probes used in our Northern analyses (Figure 3) are represented in blue.