Figure 5
From: Exonization of active mouse L1s: a driver of transcriptome evolution?
Annotation of antisense splice sites in different subfamilies of putatively active L1s and antisense intronic FL L1 insertions. A. For illustration the polypyrimidine tracts for the site SA-154 are shown. Here, the splice donor AG motif is present only in a small fraction of full-length intact elements belonging to the A subfamily (741), whereas it is intact in GF subfamily (190). "01" marks the location of AG splice acceptor motif; "-" designates the position of the polypyrimidine tract. Exemplary Motif1 and Motif2 sequences, containing the functional SA-154 splice site, are evidenced by mapping of cDNAs (AK145348, BG144807, respectively) to the corresponding genomic locations containing L1s (NCBIm35). B. Conservation of antisense GT/AG splice motifs in potentially active L1s. C. Conservation of antisense GT/AG splice motifs in antisense intronic FL L1 insertions. "n = " indicates the number of annotated L1s/monomers. Legend: cDNA-identified splice sites.