Comparison of PCR products amplified with two different DNA polymerase systems. A total of 100 ORFs (50 ORFs per enzyme mix), ranging from 300 to 4,000 bp in size, were amplified. Electrophoretic analysis of 10 representative ORFs amplified using either the supplier I (left panel) or the Phusion™ High-Fidelity DNA Polymerase (Finnzymes) (right panel). One-tenth of each reaction product of first and second step ORF amplification were loaded adjacent to each other on an analytical agarose gel. According to the lane number the expected ORF sizes and accession numbers of first-step PCR templates are as follows: 1: 759 bp, BC100921; 2: 1125 bp, BC093648; 3: 1554 bp, BC104948; 4: 3198 bp, BC117368; 5: 651 bp, BC105131; 6: 1653 bp, BC109061; 7: 2400 bp, BC113416; 8: 1737 bp, BC117320; 9: 1854 bp, BC101755; 10: 720 bp, BC113739.'M' indicates the molecular weight marker lanes.