Skip to main content

Table 3 Success rates of ORF cloning in dependence on the template used

From: The full-ORF clone resource of the German cDNA Consortium

   PCR BP reaction Entry clones
ORFs targeted # total # posa % # posb % # +/- stop codonc # + stop codond # - stop codone
cDNA clones 1712 1571 92 1477 94 1355 63 55
EST clones 368 341 93 314 92 290 9 13
Primary cDNA 156 85 54 76 89 69 1 3
Sum 2236 1997 89f 1867 93g 1714 73 71
  1. a Number of ORFs with expected product size obtained by PCR.
  2. b Number of ORFs with expected product size detected by colony-PCR of entry clones.
  3. c ORFs represented by at least one sequence verified entry clone with and without a stop codon.
  4. d,e At least one sequence verified entry clone with(d) or without(e) a stop codon was obtained for these ORFs.
  5. f,g Cumulative success rates of PCR reaction (f) and BP cloning (g)