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Table 3 Success rates of ORF cloning in dependence on the template used

From: The full-ORF clone resource of the German cDNA Consortium

  

PCR

BP reaction

Entry clones

ORFs targeted

# total

# posa

%

# posb

%

# +/- stop codonc

# + stop codond

# - stop codone

cDNA clones

1712

1571

92

1477

94

1355

63

55

EST clones

368

341

93

314

92

290

9

13

Primary cDNA

156

85

54

76

89

69

1

3

Sum

2236

1997

89f

1867

93g

1714

73

71

  1. a Number of ORFs with expected product size obtained by PCR.
  2. b Number of ORFs with expected product size detected by colony-PCR of entry clones.
  3. c ORFs represented by at least one sequence verified entry clone with and without a stop codon.
  4. d,e At least one sequence verified entry clone with(d) or without(e) a stop codon was obtained for these ORFs.
  5. f,g Cumulative success rates of PCR reaction (f) and BP cloning (g)
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BMC Genomics

ISSN: 1471-2164

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