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Table 4 ABI 3730 XL run module for SNaPshot HICF 4-color fingerprinting using POP-7 polymer in a 50 cm capillary

From: A first generation BAC-based physical map of the channel catfish genome

Setting

Value

Oven Temperature

63

Buffer Temperature

35

PreRun Voltage

15.0

PreRun Time

180

Injection Voltage

2.0

Injection Time

20

First ReadOut Time

200

Second ReadOut Time

200

Run Voltage

15.0

Voltage Number Of Steps

10

Voltage Step Interval

20

Voltage Tolerance

0.6

Current Stability

30.0

Ramp Delay

200

Data Delay

400

Run Time

2100