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Table 3 Real time qRT-PCR primer sequences, optimum primer concentrations and amplicon sizes for all validated genes.

From: Innate gene repression associated with Mycobacterium bovis infection in cattle: toward a gene signature of disease

Array feature/Clone ID

Gene symbol

Forward primer (5'-3')

Reverse primer (5'-3')

Amplicon size (bp)

Primer conc. (nM)

Reference gene

H3F3A

CATGGCTCGTACAAAGCAGA

ACCAGGCCTGTAACGATGAG

136

100

PCR Amplicon

ACTB

AAGCCGGCCTTGCACAT

TAACTCGAGAGCCAACGTCTCC

66

900

NBFGC_BE752490

ADAM17

TCAAAGTCGTGGTGGTAGATGG

AATTAGTCTCCAAAGCGGCTCT

188

900

NBFGC_AW656779

AKT1

GAGTACTTCAGGGCCGTCAG

GGTGATCCTGGTGAAGGAGA

160

900

PCR Amplicon

BCL2

ATGACTTCTCTCGGCGCTAC

ATGACCGAGTACCTGAACCG

244

300

PCR Amplicon

CCL1

AGGCTGGATCTGCTCCCAAAT

GGTGATGTGTGCAAGTTCACCA

152

900

BOTL0100003XA07R

CD81

TTCATGTCCTGAAGCTCCCTGT

TGAAGGCATAAGGCTGCTCGT

284

300

BOTL0100013_C12

CD84

TAAGTGGTGTGTCATGGCAGGT

GGCTGGAGGCTGAATATGACTG

103

300

PCR Amplicon

CXCR3

GAAAGCAGTGTGGACATAGCCA

CGGAACTTGACACCCACAAAG

101

900

NBFGC_BF230159

EEF1G

TGGATGCTCACTTGAAGACG

ACTGGGCCATTTTCTCACAG

222

300

PCR Amplicon

GAPDH

CTCCCAACGTGTCTGTTGTG

TGAGCTTGACAAAGTGGTCG

222

300

BOTL0100013_E07

IER5

AAGACCCCCGAGACTTCG

ACACTCTTCAAGGCGGAGAG

115

300

PCR Amplicon

IFNG

TGATGGCATGTCAGACAGCA

GGCACAAGTCATATAGCCTGACAC

51

300

PCR Amplicon

IL2

CTTGCACTCGTTGCAAACG

CAAGCTCTCCAGGATGCATACA

183

300

PCR Amplicon

IL4

GCCACACGTGCTTGAACAAA

TCTCAACAGCTTGGCAAGCA

63

300

PCR amplicon

IL8

AGGTGGTGTTTGAAGCCCAT

CACAACCTTCTGCACCCACTT

123

900

PCR Amplicon

IL16

CGCGGTTTGAAGAATGGAAC

TCACAGGTCCATCAGGCAAC

51

300

PCR Amplicon

MCL1

AGGTGACTGAAAGGCCTGTCTC

CAACATGTGCCTCTTCTCCCT

244

900

BF775342 NFATC4

NFATC4

AACCACTGCCCCTCTCTGAAAC

CCTCGACCCCAGATCACAAAGA

107

300

BOTL0100003XF01R

NFKB1

ATACTGAACAATGCCTTCCGG

CACGTCAATGGCCTCAGTGTAG

135

300

BOTL0100013_G05

PHB2

GGCGGCGCGGATGT

AGGTTATATCAAGCTACGCAAGATCC

65

900

NBFGC_AW335987

PRKCB1

ATCGAGAGGGAGGTCCTCAT

GGTCTTGGTCTTCTGCTTGC

141

300

PCR Amplicon

RPL19

AATGCCCGAGAAGGTAACCTG

GGATATGTTCCATGAGGATCCG

164

100

NBFGC_AW669767

RPS6KB2

TGTGGAACTGGCCTATGCCTTC

AAGATGCCTTCTCGCTCCAGGT

105

300

PCR Amplicon

STK17B

ACAGGCCCTCTTGTAATGGCAC

AGCAAATCGGACACAAGCTCG

136

300

NBFGC_BE479784

TBK1

TGGACCAATTGACTGGAGTGGA

TGATCTGCCTCAAGGATGTTTG

105

300

Not represented

TLR2

CCATTGACAAGAAGGCCAT

AACCCTTCCTGCTGAGTCTCAT

107

900

Not represented

TLR4

CGAGAGCACCTATGATGCCTTT

ATGGCCACCCCAGGAATAAA

144

900

PCR Amplicon

TNF

TCTACCAGGGAGGAGTCTTCCA

GTCCGGCAGGTTGATCTCA

68

300

  1. Clone IDs were obtained from the Center for Animal Functional Genomics (CAFG) website [51].