Skip to main content

Table 3 Validation of microarray data using quantitative PCR

From: Vascular microarray profiling in two models of hypertension identifies caveolin-1, Rgs2 and Rgs5 as antihypertensive targets

Affymetrix ID

Accession No.

Gene name

Fold-change of signal Microarray

Fold-change of signal qPCR

Validate

   

SHR

ACTH

SHR

ACTH

SHR

ACTH

1387128_at

NM_130779.1

Adcy3, adenylate cyclase 3

0.70*

0.67*

1.01

0.37*

No

Yes

1370131_at

NM_031556.1

Cav1, caveolin 1

2.88*

2.51*

10.76*

2.53*

Yes

Yes

1367600_at

NM_022531.1

BC061872

Des, desmin

0.38*

0.87

0.58*

0.76

Yes

Yes

1371166_at

NM_021838.2

AJ011116.1

NOS3, eNOS, endothelial nitric oxide synthase

1.26

1.00

0.89

0.72

Yes

Yes

1373499_at

BF287008

U77829

Gas5, growth arrest specific 5

0.77*

1.59*

0.18*

1.55*

Yes

Yes

1386881_at

NM_012588.1

Igfbp3, insulin-like growth factor binding protein 3

1.22

3.38*

0.87

2.07*

Yes

Yes

1375857_at

AW917760

XM_220031

Myof, myoferlin

1.64*

0.90

2.52*

1.01

Yes

Yes

1371776_at

AA819268

NM_013005

Pik3r1, PI3 kinase regulatory subunit polypeptide 1

13.21*

1.12

2.30*

2.04*

Yes

No

1368144_at 1387074_at

AF321837.1

AY043246.1

Rgs2, regulator of G-protein signaling 2

1.12 0.84

0.46* 0.52*

0.63*

0.19*

No

Yes

1370228_at

X77158

Tf, transferrin

1.44

3.40*

0.65*

1.80*

No

Yes

1370023_at

NM_021654.1

M76532

Gja4, connexin37, Cx37

0.96

0.82

0.79

1.06

Yes

Yes

1368473_at

NM_019280.1

BC070935

M83092

Gja5, connexin40, Cx40

1.09

0.95

0.73

1.36

Yes

Yes

1372002_at

AI411352

NM_012567

Gja1, connexin43, Cx43

1.27

0.96

0.36*

1.07

No

Yes

  1. * significantly different from WKY.
  2. Two microarray values for Rgs2 represent 2 different Affymetrix probesets.