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Table 3 Results from Real-time PCR

From: Gene expression profiles in testis of pigs with extreme high and low levels of androstenone

Gene

Real-time PCR fold change

 

ACTB

18S

CYP17A1

2.28 (p = 0.0015)

2.87 (p = 0.001)

CYB5

2.24 (p = 0.08)

2.84 (p = 0.025)

FTL

1.88 (p = 0.078)

2.58 (p = 0.0026)

EEF1A1

0.95 (p = 0.77)

1.30 (p = 0.23)

  1. Real-time PCR was used to confirm expression variations of selected genes from the microarray study. Normalisation of the values was performed by using ACTB and 18S as control genes. Three of four examined genes were verified and one was contradicted.