Gene expression profiles in testis of pigs with extreme high and low levels of androstenone

BMC Genomics

Table 4 Results from the rcPCR bootstrap statistics (x4000), Duroc

Gene	Fold	Log10 Fold change	Bias	Std error	P value
SMPD1	1.31	0.12	0.0008	0.09	0.097
StAR	5.4	0.73	0.0004	0.10	0.000
SULT2A1	2.3	0.37	0.0007	0.08	0.000
HSD17B4	1.7	0.24	-0.0009	0.09	0.006
AKR1C4	1.7	0.23	0.0013	0.09	0.003
CYP11A1	2.4	0.38	0.0000	0.09	0.000

rcPCR was used to confirm expression variations of selected genes from the microarray study. Fold changes are calculated relative to baseline, which is the group of low androstenone (DL) in this case, and adjusted for the housekeeping gene HPRT. Five of six examined genes were verified and one was contradicted.

ISSN: 1471-2164