Figure 5

S. mansoni Vasohibin orthologs and expression along the life cycle. A: Multiple sequence alignment of SmVASLv6a (the longer isoform) and several orthologs. B: Maximum Likelihood tree constructed from the alignment of SmVASLv6a and other vasohibins found in public databases. The S. mansoni branch is represented in red. Numbers next to the branches represent bootstrap values (in 1000 samplings). C: Real time RT-PCR using total RNA samples from egg, miracidium, cercaria, schistosomulum or adult and primers for SmVASL. Relative fold change was calculated by comparing the Ct value for each sample to Ct values for alpha-tubulin (internal standard).