Table 3 Predicted ORFs in the sequence inserted into ORF 32 and their highest BlastX matches. Nucleotide numbering is from the 5' end of the insertion sequence; nucleotide 8685 corresponds to nucleotide 14790 in the BJ1 genomic sequence. The sequence at the site of insertion was tgctcggtcgtcaa/CGACGCCGACGACGGCGA; lower case variant, upper case BJ1 ORF 32. Orfs are in the forward direction with respect to the virus genome unless indicated by a - sign. * indicates a truncated ORF because of incomplete sequencing (V10) or the insertion event itself (V1 and ORF32) aa indicates the number of amino acids.
From: Sequence analysis of an Archaeal virus isolated from a hypersaline lake in Inner Mongolia, China
ORF | Position | Size | Homologs (% Identity) | |
|---|---|---|---|---|
| Â | Start | Stop | (aa) | Â |
V10* | 2 | 277 | * | 67% – ornithine cyclodeaminase Natronomonas pharaonis DSM 2160 |
V9- | 749 | 351 | 132 | 36% – hypothetical protein VNG6157H Halobacterium sp. NRC-1 |
V8- | 1910 | 843 | 355 | 70% – cell division protein pelota Natronomonas pharaonis DSM 2160. |
V7- | 3051 | 1936 | 371 | 28% – hypothetical protein NP4342A Natronomonas pharaonis |
V6 | 3346 | 3753 | 135 | 38% – hypothetical protein rrnAC2062 Haloarcula marismortui |
V5 | 3912 | 4685 | 257 | 38% – Alpha/beta hydrolase fold protein Ralstonia eutropha JMP134 |
V4 | 4747 | 5058 | 103 | 75% – hypothetical protein HQ2797A Haloquadratum walsbyi DSM 16790 |
V3- | 7408 | 5900 | 502 | 73% – RtcB-like protein 1 Natronomonas pharaonis DSM 2160 |
V2- | 7934 | 7503 | 143 | 61% – hypothetical protein NP3986A Natronomonas pharaonis DSM 2160 |
V1* | 8326 | 8684 | 119* | 64% – 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase) Haloferax volcanii |
32* | 8685 | 9059 | * | 100% Phage BJ1 hypothetical protein |