Skip to main content
Figure 1 | BMC Genomics

Figure 1

From: Analysis of the hybrid proline-rich protein families from seven plant species suggests rapid diversification of their sequences and expression patterns

Figure 1

Evolutionary relationships and expression patterns of potato HyPRPs. Left: an unrooted consensus phylogenetic tree (cladogram) of nucleotide sequences encoding the conserved C-terminal domains of HyPRPs from potato (Solanum tuberosum), constructed by the NJ method using programs from the PHYLIP package (see Materials and Methods). Bootstrap values above 50 % (from 500 replicates) are shown above the individual branches, numbers below branches denote bootstrap values of a ML tree from the same input data. Gene names are color-coded according to the composition of the N-terminal domains of the encoded proteins (see Table 2). "Standard" proline-rich N-terminal domains (more than 20 % of Pro, Pro to Gly ratio larger than 2) are shown in black, glycine-rich N-terminal domains (more than 20 % of Gly, Gly to Pro ratio larger than 2) in green, N-terminal domains shorter than 10 amino acid residues in red. Among proteins with "standard" (Pro-rich) N-terminal domains, those with extremely long N-termini (over 80 residues) are shown in bold, those with increased contents of serine and threonine in italics, lysine-rich ones are underlined, hydrophobic (A, V, L, I-rich) are marked by a plus (+) sign. An asterisk denotes a truncated N-terminus. Right: expression profiles of fourteen potato HyPRP genes (see Table 1) in vegetative organs of in vitro cultured potato plants determined by semiquantitative PCR. Expression of the ef1a gene was used as the internal standard (products of PCR with 23 and 30 cycles are shown).

Back to article page