Table 2 Verification of DNA microarray-derived results with qRT-PCR*1
Translational repression factor exp | Translational repression factor stat | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
ORF | Realtime PCR | Microarray | Realtime PCR | Microarray | ||||||||
∅ | SD | n | ∅ | SD | n | ∅ | SD | n | ∅ | SD | n | |
OE2010R | 1.2 | 0.1 | 3 | 1.0 | 0.0 | 4 | 1.2 | 0.4 | 3 | 1.0 | 0.1 | 3 |
OE2055F | 1.0 | 0.1 | 3 | 1.0 | 0.2 | 4 | 1.3 | 0.1 | 3 | 1.0 | 0.1 | 3 |
OE2563R | 0.8 | 0.1 | 3 | 1.0 | 0.1 | 4 | 0.7 | 0.1 | 3 | 1.0 | 0.1 | 3 |
OE2595F | 1.2 | 0.1 | 3 | 1.0 | 0.2 | 4 | 1.0 | 0.1 | 3 | 1.0 | 0.1 | 3 |
OE3637R | 0.7 | 0.1 | 3 | 1.0 | 0.2 | 4 | 0.9 | 0.4 | 3 | 1.0 | 0.1 | 3 |
OE4674F | 1.1 | 0.1 | 2 | 0.9 | 0.1 | 4 | 0.8 | 0.2 | 3 | 0.9 | 0.0 | 3 |
OE4511R | 45.4 | 16.3 | 3 | 9.0 | 3.8 | 4 | 12.7 | 5.3 | 3 | 1.2 | 0.2 | 3 |
OE3538R | 19.6 | 2.9 | 3 | 3.5 | 2.0 | 4 | 4.3 | 1.4 | 3 | 0.9 | 0.1 | 2 |
OE4187R | 8.7 | 1.0 | 3 | 2.6 | 1.1 | 4 | 6.4 | 0.7 | 3 | 1.4 | 0.2 | 2 |
OE1119F | 3.0 | 1.0 | 3 | 4.7 | 2 | 1.4 | 0.2 | 3 | 1.2 | 0.0 | 2 | |
OE3049R | 2.2 | 0.6 | 3 | 2.7 | 1.8 | 3 | 1.6 | 0.2 | 3 | 1.2 | 0.4 | 3 |
OE1405R | 9.2 | 1.2 | 3 | 2.4 | 0.9 | 4 | 13.5 | 4.9 | 3 | 5.1 | 0.2 | 3 |
OE3470F | 1.9 | 1.0 | 3 | 0.6 | 0.2 | 4 | 7.4 | 4.1 | 3 | 8.4 | 6.1 | 3 |
OE5071F | 2.7 | 0.6 | 3 | 0.6 | 0.3 | 4 | 1.5 | 0.3 | 3 | 2.7 | 0.1 | 3 |