Re-linearization of vector pGEM-T at its cloning site. A. Simplified map of vector pGEM-T. The insert DNA of interest was cloned into position between bases 60 and 61. The primers were introduced with the DNA insert during cDNA preparation carried out in a wet lab. B. Vector sequence of pGEM-T before and after re-linearization Bases 1–198 and 2899–3015 were expressed. The omitted nucleotides are expressed as dotted lines. Additional nucleotides TA (colored in blue) were appended to the vector at position 60 during a wet-lab experimental procedure. The letters in pink boxes (bases 1 ~ 60 plus the appended T) were moved electronically to the end of the sequence for vector re-linearization.