Table 2 Cloning vectors, primers and adaptors used for ESTs in this study
From: An optimized procedure greatly improves EST vector contamination removal
Vector (cutting site) | Primer/adaptor | No. seq | Source of EST |
|---|---|---|---|
PGEM-Teasy (60–61) | primer1, primer2R, adapter1, adapter2R | 2882 | ABRC laboratories |
pTriplEx2 (571–581) | ToTom | 2318 | ABRC laboratories |
pCR21TOPO (294–295) | primer1, primer2R, adapter1, adapter2R | 5 | ABRC laboratories |
pZL1 (239–253) | Not I primer-adapter, Sal I adapter | 700 | ABRC laboratories |
pT7Blue (95–96)a | Not I primer-adapter, Sal I adapter | 130 | ABRC laboratories |
pCMV-SPORT6.1 (774–1222) |  | 3000 | trace archive at NCBI |
pT7T3-PAC (200–240) | Eco RI adaptors | 3000 | trace archive at NCBI |
pDNR-LIB (77–298) | Sfi-(dT)30; AAGCAGTGGTATCAACG CAGAGTGGCC | 3000 | trace archive at NCBI |