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Table 2 Cloning vectors, primers and adaptors used for ESTs in this study

From: An optimized procedure greatly improves EST vector contamination removal

Vector (cutting site) Primer/adaptor No. seq Source of EST
PGEM-Teasy (60–61) primer1, primer2R, adapter1, adapter2R 2882 ABRC laboratories
pTriplEx2 (571–581) ToTom 2318 ABRC laboratories
pCR21TOPO (294–295) primer1, primer2R, adapter1, adapter2R 5 ABRC laboratories
pZL1 (239–253) Not I primer-adapter, Sal I adapter 700 ABRC laboratories
pT7Blue (95–96)a Not I primer-adapter, Sal I adapter 130 ABRC laboratories
pCMV-SPORT6.1 (774–1222)   3000 trace archive at NCBI
pT7T3-PAC (200–240) Eco RI adaptors 3000 trace archive at NCBI
pDNR-LIB (77–298) Sfi-(dT)30; AAGCAGTGGTATCAACG CAGAGTGGCC 3000 trace archive at NCBI
  1. a Only 14 of the 130 ESTs cloned into pT7Blue in this test used the indicated adapter while the remaining 116 used no adapters.