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Table 5 Vector contamination in ESTs sampled from dbEST

From: An optimized procedure greatly improves EST vector contamination removal

Vector a# Contaminated ESTs b# ESTs cRatio
pT7T3D-PacI 20 3883 0.52%
pSPORT1 99 3784 2.62%
pCMVSPORT6.0 17 4784 0.36%
pME18S-FL 13 3382 0.38%
pUC18 17 1618 1.05%
pCS107 3 1198 0.25%
PBRcDNASfiIAB 5 1177 0.42%
pBluescriptSK(-) 20 2494 0.80%
pDNR-LIB 24 1817 1.32%
pOTB7 6 891 0.67%
pBluescriptIISK(+) 27 2516 1.07%
pBK-CMV 11 783 1.40%
pBluescriptSK(+) 11 713 1.54%
pExpress-1 19 1507 1.26%
pBluescriptIIKS(+) 7 689 1.02%
pcDNA3.1(+/-) 8 469 1.71%
pCMV-SPORT6.1 186 1676 11.10%
pTriplEx2 60 545 11.01%
pDONR222 20 516 3.88%
pCS108 1 362 0.28%
pT7T3D 1 559 0.18%
Total 575 35363 1.63%d
  1. aNumber of ESTs which were cloned into the indicated vector remained to be contaminated with vector.
  2. bNumber of ESTs using the indicated vector were subjected to BLAST analysis for identification of vector contamination
  3. cpercentage of vector contamination for the ESTs using the same cloning vector.
  4. dpercetage obtained by 575/35363 = 1.63%