An optimized procedure greatly improves EST vector contamination removal

BMC Genomics

Table 5 Vector contamination in ESTs sampled from dbEST

Vector	^a# Contaminated ESTs	^b# ESTs	^cRatio
pT7T3D-PacI	20	3883	0.52%
pSPORT1	99	3784	2.62%
pCMVSPORT6.0	17	4784	0.36%
pME18S-FL	13	3382	0.38%
pUC18	17	1618	1.05%
pCS107	3	1198	0.25%
PBRcDNASfiIAB	5	1177	0.42%
pBluescriptSK(-)	20	2494	0.80%
pDNR-LIB	24	1817	1.32%
pOTB7	6	891	0.67%
pBluescriptIISK(+)	27	2516	1.07%
pBK-CMV	11	783	1.40%
pBluescriptSK(+)	11	713	1.54%
pExpress-1	19	1507	1.26%
pBluescriptIIKS(+)	7	689	1.02%
pcDNA3.1(+/-)	8	469	1.71%
pCMV-SPORT6.1	186	1676	11.10%
pTriplEx2	60	545	11.01%
pDONR222	20	516	3.88%
pCS108	1	362	0.28%
pT7T3D	1	559	0.18%
Total	575	35363	1.63%^d

^aNumber of ESTs which were cloned into the indicated vector remained to be contaminated with vector.
^bNumber of ESTs using the indicated vector were subjected to BLAST analysis for identification of vector contamination
^cpercentage of vector contamination for the ESTs using the same cloning vector.
^dpercetage obtained by 575/35363 = 1.63%

ISSN: 1471-2164