Table 6 PCR primers and conditions for identification of SNPs in TLR2.
Exon | Primer name | Sequence, 5' → 3' | Tann, °C | Amplicon size, bp |
|---|---|---|---|---|
TLR2 PROMOTER | Â | Â | Â | Â |
Part 1 | Forward | CCCTTGAATGTATTTGTCACTTCC | 60 | 664 |
| Â | Reverse | TCCATATTTTTGAGAATCTGACTGA | Â | Â |
Part 2 | Forward | TGCAAGGATTACAGCAGATTTTC | 59 | 579 |
| Â | Reverse | AAAATCATGTATTTACCCACAACG | Â | Â |
Part 3 | Forward | CGTACAAAGATGTGCCAGAGG | 59 | 586 |
| Â | Reverse | CCAGTGTTCTTGCCTGGAGA | Â | Â |
Part 4 | Forward | GGGGCCTTTAATGAGACCTG | 60 | 445 |
| Â | Reverse | GCCCTTCCTAAATTGAGATCAGA | Â | Â |
Part 5 | Forward | GTGCCTTTGCAATGAGGACT | 60 | 496 |
| Â | Reverse | GCCTGAAGAGCAGATGAGGA | Â | Â |
TLR2 GENE | Â | Â | Â | Â |
Part 1 | Forward | GCCATGATGTCAAACACAGTCA | 58 | 613 |
| Â | Reverse | AGCACTGATCTCAAGCTCCTCA | Â | Â |
Part 2 | Forward | CAAAACACTTGGGGAAACATCT | 59 | 611 |
| Â | Reverse | TTTTAACTCTGCCTGTGAGTGGA | Â | Â |
Part 3 | Forward | ACTGTACCCATGATGGAATTGG | 61 | 643 |
| Â | Reverse | ACGGGTAAGAAGGAGGCATCT | Â | Â |
Part 4 | Forward | CACAGTTTAACCCAGTGCCTTC | 59 | 633 |
| Â | Reverse | CATGAGGTTCTCCACCCAGTAG | Â | Â |
Part 5 | Forward | CGGACTGTGGTACATGAAGATG | 60 | 643 |
| Â | Reverse | TCAGCATCAGTTCTTCCAATGA | Â | Â |