Table 7 PCR primers and conditions for identification of CARD15 single-nucleotide polymorphisms.
Exon | Primer name | Sequence, 5' → 3' | Tann, °C | Amplicon size, bp |
|---|---|---|---|---|
CARD15 PROMOTER | Â | Â | Â | Â |
Part 1 | Forward | CCCCACTTCTATCCTCTGGACT | 58 | 640 |
| Â | Reverse | AGCAGAGGAAATCTCTCAGCAG | Â | Â |
Part 2 | Forward | GACTTGGCCATGCCTACTCTAA | 59 | 601 |
| Â | Reverse | GAAACTGAGGCAGCCAGGTAG | Â | Â |
Part 3 | Forward | TAGACACAGAGTGCTGGCAAGA | 58 | 625 |
| Â | Reverse | CCCGCAAAGATCATTAGGTTTA | Â | Â |
Part 4 | Forward | TCTTTCCTATCTGCAGCTGTCC | 59 | 632 |
| Â | Reverse | AAGTCCCATCTCTGTCCTAGCC | Â | Â |
CARD15 GENE | Â | Â | Â | Â |
1 | Forward | TGTAGACAGACGCTGGAGTTCCTCT | 62 | 322 |
| Â | Reverse | ATGCACTGACTCCCATCTACTGCTC | Â | Â |
2 | Forward | GAGAAGCCCTGCCCTGACCT | 63 | 599 |
| Â | Reverse | ACTATGACCCACATCTCCCCACAG | Â | Â |
3 | Forward | TGCATCTTACCATGCAGATGTTTTTC | 62 | 392 |
| Â | Reverse | GAGACGCTGAGTTTTACGGAGGTTT | Â | Â |
4 (part1) | Forward | CTTGTTAGTGGAGAGCCAGGAC | 58 | 546 |
| Â | Reverse | AACAGCAGTGTTCGAAGAGCA | Â | Â |
4 (part2) | Forward | TTCTCTTCGTCTTCCCATTTAGC | 59 | 506 |
| Â | Reverse | GGACACCATCCAGGAGAAGAC | Â | Â |
4 (part3) | Forward | CATCGAACTGTACCTGAGGAAGC | 60 | 594 |
| Â | Reverse | AGGAACAATTTGGGCAGCAC | Â | Â |
4 (part4) | Forward | TCAGACATCTCTTCCAAGATCACA | 59 | 680 |
| Â | Reverse | ACTCCTGGCTCCCAGCATAA | Â | Â |
56 | Forward | GGGTTAAGCAAAAGTCTCTGTGG | 59 | 595 |
| Â | Reverse | AATGAGACACTGTCCTTGTTTTCAG | Â | Â |
7 | Forward | CAGGTCTTGGGAGCAGTAAGG | 59 | 375 |
| Â | Reverse | ACATCCTGAGCTTCCTGTTTATTG | Â | Â |
8 | Forward | CTCACTTGCTGGGACCTGAGT | 59 | 285 |
| Â | Reverse | TCCCTCCTCACACTGGCTTC | Â | Â |
9 | Forward | AACGATTAGTCTGAAATGGAGCAG | 58 | 300 |
| Â | Reverse | TACACACACATCAGCTTCCACAG | Â | Â |
10 | Forward | CCTTACACTTTGCTTGACCTGTTT | 58 | 472 |
| Â | Reverse | ACCCCGAAAGATTGTTTTCTAGG | Â | Â |
11 | Forward | GAATTCATTGGGAATCTCAGACAG | 59 | 355 |
| Â | Reverse | CAGGACTAGAGGTCTGAGCCATAA | Â | Â |
12(part1) | Forward | ACAGGTTTACAAAGCAGCATCTTC | 59 | 578 |
| Â | Reverse | ACCACTCAACCTGATGGATGAC | Â | Â |
12(part2) | Forward | GGCTGGTCTCAAGTCAAGCTG | 60 | 619 |
| Â | Reverse | GCACAAACAAAACCAAACCATC | Â | Â |
12(part3) | Forward | GAACAACTCTGTATCCAAATGCAAC | 59 | 641 |
| Â | Reverse | ACGCTGGGCTAGACTTCTCTAAAC | Â | Â |
12(part4) | Forward | TCTCCTTGAAGGGAGGAGACAT | 59 | 509 |
| Â | Reverse | CACAACGCTGTAAATCAACCATAC | Â | Â |
12(part5) | Forward | GGCCTTGGTGAAATAATTCTTAGC | 58 | 588 |
| Â | Reverse | CAGCAACATGGATGGATCTAGAAA | Â | Â |