Table 8 The tetra-primer Amplification Refractory Mutation System-PCR primers and conditions for genotyping CARD15 SNPs.
| Position | Primer Name | Sequence, 5' → 3' | Tann, °C | Amplicon size, bp |
|---|---|---|---|---|
| c.2886-14A>G |
Forward inner primer Reverse inner primer Forward outer primer Reverse outer primer |
CAAGCATCCTCAAAGTTCACCATTA TG GTGGTTGTTAGACAGCCTAGAGAGGAAT AT CCTGGCACTAAAATGCACGTATTTTTAT AGTTATACGTTGGAAAACTGAGGCTCAG | 60°C |
405bp(outer) 300 bp (allele 'A') 150 bp (allele 'G') |
| c.3020A>T |
Forward inner primer Reverse inner primer Forward outer primer Reverse outer primer |
GAGGAAATTGAGAAACTCAGCCAG CA AAACATCAGAGCAAGAGTCTGGTATG CA CTGACAGAGGGTAAAACCTGCACAGACG CTGTGCCAGAACAAAGGTGACCTATTG | 59°C |
310bp (outer) 197 bp (allele 'A') 166 bp (allele 'T') |
| c.4500A>C |
Forward inner primer Reverse inner primer Forward outer primer Reverse outer primer |
AGAGACGCAAGCAGGCCCCTGGGCCG CA CCCTGGAGACACTTGGAGAGAATGGG GG AGCAGTGTTTAGAAATAGCCTCGCAAT AGAGAACCCACACACATGCCCTTACTG | 64°C |
400bp (outer) 250 bp (allele 'A') 150 bp (allele 'C') |
| c.4950C>T |
Forward inner primer Reverse inner primer Forward outer primer Reverse outer primer |
TGTATCCATTCAATATATAATACATTG GC GGCTAGCAAATGTATTATGAGGTG AA TTCATTTGTTTGAATTTATTTTATTGAGG GAATGTAACACAAAGGAACATATCTACAA | 59°C |
341bp (outer) 226 bp (allele 'T') 169 bp (allele 'C') |