Position | Primer Name | Sequence, 5' → 3' | Tann, °C | Amplicon size, bp |
---|---|---|---|---|
c.2886-14A>G | Forward inner primer Reverse inner primer Forward outer primer Reverse outer primer | CAAGCATCCTCAAAGTTCACCATTA TG GTGGTTGTTAGACAGCCTAGAGAGGAAT AT CCTGGCACTAAAATGCACGTATTTTTAT AGTTATACGTTGGAAAACTGAGGCTCAG | 60°C | 405bp(outer) 300 bp (allele 'A') 150 bp (allele 'G') |
c.3020A>T | Forward inner primer Reverse inner primer Forward outer primer Reverse outer primer | GAGGAAATTGAGAAACTCAGCCAG CA AAACATCAGAGCAAGAGTCTGGTATG CA CTGACAGAGGGTAAAACCTGCACAGACG CTGTGCCAGAACAAAGGTGACCTATTG | 59°C | 310bp (outer) 197 bp (allele 'A') 166 bp (allele 'T') |
c.4500A>C | Forward inner primer Reverse inner primer Forward outer primer Reverse outer primer | AGAGACGCAAGCAGGCCCCTGGGCCG CA CCCTGGAGACACTTGGAGAGAATGGG GG AGCAGTGTTTAGAAATAGCCTCGCAAT AGAGAACCCACACACATGCCCTTACTG | 64°C | 400bp (outer) 250 bp (allele 'A') 150 bp (allele 'C') |
c.4950C>T | Forward inner primer Reverse inner primer Forward outer primer Reverse outer primer | TGTATCCATTCAATATATAATACATTG GC GGCTAGCAAATGTATTATGAGGTG AA TTCATTTGTTTGAATTTATTTTATTGAGG GAATGTAACACAAAGGAACATATCTACAA | 59°C | 341bp (outer) 226 bp (allele 'T') 169 bp (allele 'C') |