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Table 7 Real-time PCR validation of microarray negative results

From: Differential gene expression profile in pig adipose tissue treated with/without clenbuterol

Gene* (Clone No. on Microarray)

Hog 1/hog 2

Sow 1/sow 2

Hog 3/hog 4

Sow 3/sow 4

 

Change (test/control)

P value

Change (test/control)

P value

Change (test/control)

P value

Change (test/control)

P value

SCD (rpfat_16685)

1.56

0.1680

0.77

0.2119

1.49

0.0822

1.23

0.9943

HSL (rpfat_11096)

1.29

0.0679

1.11

0.2989

1.29

0.3462

1.06

0.5294

PMP22a (rpfat_18575)

1.15

0.6198

1.16

0.3178

1.24

0.6900

1.46

0.1175

PHPT1b (rpfat_15312)

0.75

0.1218

0.95

0.5811

1.24

0.4694

1.46

0.4869

  1. * The full names of each gene are listed in Tables 2-5.
  2. a : PMP22: peripheral myelin protein 22
  3. b :PHPT1: Phosphohistidine phosphatase 1
  4. Three genes (HSL, PMP22 and PHPT1) were confirmed by real-time PCR.