Figure 1

Diagrammatic illustration showing cloning strategy of buffalo Smoc -1. Smoc-1 structure representing 5'/3'UTRs, domain organization and nucleotide boundary of each exon is shown in (A). The strategy for isolation of the Smoc-1 is given in (B). Different fragments generated by end point PCR (blue) and RACE (pink) used to deduce the full length CDS are shown along with their nucleotide boundaries. Clone I covered nucleotides 318–1580; clone II, 119–1603; clone III, 1461–2473; clone IV, 2307–3328; and clone V, 2435–3428; clone VI, 1–649; clone VII, 1407–1915 and clone VIII, 2435–3474. Two transcript variants of Smoc-1 with their 3'UTR length variation are shown. Poly(A) tails for both the variants, -01 (3474 bp) and -02 (1934 bp) are marked by arrows in 'A'.