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Figure 12 | BMC Genomics

Figure 12

From: Bioinformatic evaluation of L-arginine catabolic pathways in 24 cyanobacteria and transcriptional analysis of genes encoding enzymes of L-arginine catabolism in the cyanobacterium Synechocystis sp. PCC 6803

Figure 12

Slot-blot transcript analysis of the genes encoding the putative enzymes of the L-arginine deiminase pathway in Synechocystis sp. PCC 6803. Synechocystis sp. PCC 6803 cells were grown for 24, 48, or 72 h with nitrate or L-arginine as sole N-source and with a constant illumination of 50 photons m-2 s-1. Steady state transcript pools were investigated with gene-specific probes of equal length and equal GC % content to assure equal labeling with Dig-dUTP. An rnpB-specific probed was used to assure equal loading. The figure allows for the direct comparison of transcript levels between cells grown with L-arginine to that grown with nitrate.

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