Pancreatic cancer gene expression. In this example, our goal was to retrieve additional expression data from a specific anatomical site for genes differentially regulated in neuroendocrine tumours (PEN). 1. Filter: one can restrict the query by clicking on the "Filters" node on the left, expanding the filter section "GENES DIFFERENTIALLY EXPRESSED IN:" to restricted the query for genes differentially expressed in "Neuroendocrine tumours (PEN) vs islet cell". Next, one can expand the filter section "EXPRESSION PATTERNS:" and restrict the query further to genes having expression profiles in pancreas and tumour by selecting. "eGenetics/SANBI EST anatomical system data" for "pancreas" (eGenetics/SANBI EST anatomical system data -> alimentary -> pancreas) and the "eGenetics/SANBI EST pathology data" for "tumour" (eGenetics/SANBI EST pathology data -> neoplasia -> tumour). 2. Attributes: one can click on the "Attributes" node on the left and start choosing attributes on the right. In this example, one can choose the "General Gene Features" attribute page, expand the attribute section "PANCREATIC EXPRESSION" to remove "Differential Expression Analysis" and "Direction of Regulation" options selected by default, expand the attribute section "EXPRESSION:" and select the "eGenetics/SANBI" under the "Source of Expression Data". 3. Results: one can preview the results by clicking the Results button on the toolbar.