Figure 12

Immunohistochemistry data. In this example, the goal was to show how to obtain immunohistochemistry data for the E-cadherin gene. 1. Filter: one can restrict the query by clicking on the "Filters" node on the left, expanding the filter section "GENE RELATED ACCESSIONS:" using the "+" box, choosing the "ID" list limit simply by clicking on the left checkbox and selecting the "HGNC Symbol(s)" and typing in the E-cadherin gene symbol "CDH1". 2. Attributes: one can click on the "Attributes" node on the left and start choosing attributes from the "General Gene Features" page. In this example, one can expand the "PANCREATIC EXPRESSION:" section, check "Ensembl Gene ID", "Specimen or Cell type", "Platform" under "Expression Attributes" and "Study" under "Origin of data". Once can also expand the "GENE:" section to check "HGNC Symbol" under the "External References (max 3)" and "ANTIBODIES:" to check "Antibody ID". 3. Results: one can click the "Results" button to view the results. The antibody ID for "CDH1" is displayed with a hyper-link to the Human Protein Atlas where immunohistochemistry data for normal and cancer tissues, as well as for cell lines, can be displayed.