Figure 1

vRLGS in the Conime interface. Using the March 2006 freeze of the human genome we generated a vRLGS profile for the enzyme combination NotI-EcoRV-HinfI and loaded it into the Conime user interface along with the human master profile. A) Three veiwports in the Conime interface. The master profile is seen in the bottom left and the virtual profile is shown on the bottom right. The upper panel is a composite image of the two with the virtual profile contributing red and the master profile contributing blue. Areas of spot overlap between the two profiles appear as dark blue. B) Zoomed in view of the gels in A). Spots 38, 45, 46, and 48 are known sequences and are labeled on both the master profile and virtual profile with their overlap shown in the upper panel. The arrow in all three profiles indicates the spot of interest on the master profile, the top candidate sequence on the virtual profile, and their overlap. To the right is shown a window that can be selected to provide information about the virtual spot of interest (clicking on the spot with the arrow). This spot of interest is found at chr16:86976875.