Application of vRLGS to the mouse genome with alternative enzyme combinations. A) A mouse vRLGS profile was generated for the enzyme combination NotI-PstI-PvuII (lower right) and compared with an actual gel (lower left). Spots of interest, previously mapped in BXD recombinant inbred strains are labeled on the actual gel and candidate vRLGS spots are labeled that are located in the same genomic region as the genetically mapped spots. The circles in the upper panel indicate the overlap between the actual and virtual spots of interest. B) Same as in A) except using the enzyme combination NotI-PvuII-PstI. The spots of interest are genetically mapped in a BSS interspecific backcross.