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Figure 2 | BMC Genomics

Figure 2

From: Discovery of novel alternatively spliced C. elegans transcripts by computational analysis of SAGE data

Figure 2

Validation RT-PCR experiments. Left panel: the results of RT-PCR experiments for eight selected exon skipping candidates are shown. Lanes 1–8 correspond to the genes C33G3.4, C52E4.6a, F27D4.4, T01G5.1, T05B4.1, T14G10.1, W04G5.9 and Y49F6B.8. PCR products of the expected sizes (400 – 600 bp) were observed for four genes. *DNA sequencing did not confirm one of the candidates (C33G3.4, lane 1 on the left panel). Right panel: the results of the intron retention analysis. The positions of the PCR product for normally spliced isoforms (empty arrows) and isoforms with intron retention (black arrows) are shown. Lanes 1–10 correspond to the genes B0041.3, C08B6.13, C14C6.5, C24G6.3, D1054.10, F07C6.2, R09E10.3, T23G7.5, W01A8.3 and Y116A8C.30. * Additional RT-PCR experiments with oligo(dT) primer followed by DNA sequencing disproved three candidates (C08B6.13, R09E10.3 and W01A8.3).

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BMC Genomics

ISSN: 1471-2164

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