Time-course of transcription factor induction during sucrose-dependent atrazine protection. (A) Zinc finger protein Zat12 (At5g59820), (B) bZIP family protein AtbZIP60 (At1g42990), (C) Sigma factor binding protein SIB1 (At3g56710), (D) AP2 domain-containing TF (At3g61630). Plantlets were transferred during 4, 8, 12 or 16 h on mannitol as osmotic control, mannitol plus atrazine as herbicide treatment and sucrose plus atrazine as protective treatment. RNA from these plantlets was extracted and used for qRT-PCR analysis. The continuous line represents log2(ratio) of relative expression between sucrose plus atrazine treatment and the physiological control, while the discontinuous line represents log2(ratio) of relative expression between mannitol plus atrazine treatment and the physiological control.